产品介绍

产品信息(Information)：

背景介绍(Background)：

This protein is a pleiotropic cytokine with roles in several different systems. It is involved in the induction of hematopoietic differentiation in normal and myeloid leukemia cells, induction of neuronal cell differentiation, regulator of mesenchymal to epithelial conversion during kidney development, and may also have a role in immune tolerance at the maternal-fetal interface. Alternatively, spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Mar 2012]

Leukemia inhibitory factor (LIF) is a multi-functional cytokine of the interleukin-6 (IL-6) superfamily. Initially identified as a factor that inhibits the proliferation of murine myeloid leukemia cells, LIF displays a wide variety of important functions in a cell-, tissue- and context-dependent manner in many physiological and pathological processes, including regulating cell proliferation, pluripotent stem cell self-renewal, tissue/organ development and regeneration, neurogenesis and neural regeneration, maternal reproduction, inflammation, infection, immune response, and metabolism. Emerging evidence has shown that LIF plays an important but complex role in human cancers. While LIF displays a tumor suppressive function in some types of cancers, including leukemia. LIF is overexpressed and exerts an oncogenic function in many more types of cancers. Further, targeting LIF has been actively investigated as a novel strategy for cancer therapy. Extracellular LIF stimulates JAK/STAT, MAPK, and PI3K signaling, forming a 1:1:1 ternary complex. Intracellularly, both chains of the LIF receptor (LIFRβ and gp130) are bound to JAK1, the tyrosine kinase that initiates the signaling cascade. JAK1 phosphorylates five tyrosines on each receptor chain, four of these are docking sites for the transcription factor STAT3 (allowing stimulation of the JAK/STAT signaling pathway), whilst the fifth is a docking site for SHP-2 (which stimulates the MAPK pathway), and SOCS3 (which negatively regulates both the JAK/STAT and MAPK pathways). LIF stimulates a mixture of differentiation, survival, and renewal programs, the balance of which determines the cell's fate. In embryonic stem (ES) cells, signaling is skewed towards survival and self-renewal.

图 LIF信号通路

制剂(Formulation)：

Lyophilized from 0.22 μm filtered solution in PBS，5%mannital，0.01% Tween80 pH7.4. 

质量控制(Quality Control)：

Purity: ≥ 95% as determined by reducing SDS-PAGE.    

Endotoxin: ≤ 10 EU/mg  as determined by LAL test.

保存(Storage)：

Use a manual defrost freezer and avoid repeated freeze - thaw cycles.
12 months from date of receipt, -20 to -70℃ as supplied.
1 month, 2 to 8℃ under sterile conditions after reconstitution.
6 months, -20 to -70℃ under sterile conditions after reconstitution.

FOR RESEARCH USE ONLY

参考文献：

[1] Zhang C, et al. 2021. Pharmacol Ther. 221:107754.

[2] Nicola NA, et al. 2015. Cytokine Growth Factor Rev. 26(5):533-44.