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产品介绍
Monoclonal Anti-Human CD3 Antibody
Mouse IgG2a（HPLC verified）
●Ultra-low endotoxin
●Ultra-high purity
●Ultra-high bioactivity
Catalog# / Size | C1103-500μg |
Clone | OKT3 |
Isotype | Mouse IgG2a, κ |
Endotoxin | Less than 0.001 EU per μg determined by LAL gel clotting assay. |
| Purity | >99% as determined by SDS-PAGE >99% as determined by SEC-HPLC |
| Antibody Type | Monoclonal |
| Expressed Host | Stable CHO cell line |
Application | T-cell activation |
Product Details
Reactivity | Human |
Concentration | 1.0 mg/mL |
Form | Liquid |
Antibody Type | Monoclonal |
Formulation | Supplied as 0.2 μm filtered solution in PBS, pH7.4. |
Storage instructions | Shipped at 4°C. Stored at 4°C for 12 months. |
Other Names | T3,CD3ε |
Description
CD3ε is a 20 kD chain of the CD3/T cell receptor (TCR) complex, which is composed of two CD3 ε, one CD3γ, one CD3δ, one CD3ζ (CD247), and aT cell receptor (α/β or γ/δ) heterodimer.
It is a specific marker for T lymphocytes, NK T cells, and some thymocytes. Crosslinking of TCR initiates an intracellular signaling cascade resulting in cellular activation and proliferation.
Application References
1 、Biggs MJ, et al. 2011.J. R. Soc. Interface. 8:1462.
2 、Manevich-Mendelson E, et al. 2009. Blood 114:2344.
3、Jeong HY, et al. 2008.J. Leuckocyte Biol. 83:755.
4 、Li B, et al. 2005. Immunology 116:487.
Product Data
 | SDS-PAGE |
 | SEC-HPLC The purity of monoclonal anti-human CD3 greater than 99% as determined by SEC-HPLC. |
 | Bioactivity-FACS The purified human T cells were activated using monoclonal anti-human CD3 antibody. Cells were fluorescently stained using APC labeled anti-human CD3 antibody and labeled FITC anti-human CD56 antibody and analyzed by flow cytometry. |
 | Bioactivity-Cell based assay PBMC derived T cells were activated in the monoclonal anti-human CD3 antibody and anti-human CD28 antibody for a period of 3 days. Activation was done by coated with monoclonal anti-human CD3 antibody, with 1 µg/mL of each antibody. Cells were cultured for a total of 9 days, and viable cell number was determined. |
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