产品介绍

Monoclonal Anti-Human CD28 Antibody humanized IgG4  （HPLC verified）

●Ultra-low endotoxin 
●Ultra-high purity

●Ultra-high bioactivity

Product Details      

T-cell-specific surface glycoprotein CD28 is a 44kD disulfide-linked homodimeric type I glycoprotein.It is a member of the immunoglobulin superfamily and is also known as T44 or Tp44.CD28 is expressed on most T lineage cells,NK cell subsets,and plasma cells.The binding of CD28 to its ligands CD80 and CD86 transfers a costimulatory signal to the T cell.Activation and proliferation of T cells can be induced by interaction of the T cell receptor(TCR) with peptide-MHC complexes expressed on the surface of antigen presenting cells(APC).T cell activation can also be achieved by agonistic stimulation with the CD3 antibody binding to the TCR complex and can be enhanced by costimulation with CD28 antibody.

Application References

1、Magatti M, et al. 2008. Stem Cells 26:182.

2、Calea-Lauri J, et al. 1999. J. Immunol. 163:62.

3、Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York.

4、Nunes J, et al. 1993. Biochem. J. 293:835.

Product Data

SDS-PAGE Monoclonal anti-human CD28 antibody on SDS-PAGE under reducing (R) and non-reducing(NR) conditions.  The gel  was  stained  with  Coomassie  Blue.The  purity of  the protein is greater than 99%.

SEC-HPLC The purity of monoclonal anti-human CD28 antibody was greater than 99% as determined by SEC-HPLC.

Bioactivity-FACS The purified  human  T  cells  were  activated  using monoclonal  anti-human  CD3 antibody and monoclonal anti-human  CD28 antibody.  Cells  were  fluorescently stained using APC labeled anti-human CD25 antibody and labeled FITC anti-human CD69 antibody and analyzed by flow cytometry.

Bioactivity-Cell based assay PBMC derived T  cells were activated in the  monoclonal anti-human CD3 antibody and anti-human CD28 antibody for a period of 3 days. Activation was done by coated with monoclonal anti-human CD3 antibody, with  1  µg/mL  of each antibody. Cells were cultured for a total of 9 days, and viable cell number was determined.